{"schema":"pubphys.bundle/1","record":{"author":{"account_ref":null,"orcid":null},"builds_on":[],"content_schema":"pubphys.content.revision/1","content_sha256":"33180143905c75a449b1df4df6c3c9d366fc40c352776be0fd167d9cd69e8045","created":"2026-10-03T07:17:58Z","files":[],"origin":{"assisted_by":[],"kind":"seed"},"parents":["5475cf5dc5b1a72283d26dc95dc6e00d7ea4e00096a7c6492e409a1816eeb011"],"salt":"505b70fb1fad8cffbe713b6c1a7be6a03f13e5bf1bc0751073711c759c24b457","schema":"pubphys.record/2","site":"pubphys.com","target":null,"type":"revision"},"content":{"answer_type":"mechanism","assisted_by":[],"external_id":"chem.catalytic-mechanisms.nitrogenase-n2-binding","kind":"well-posed","literature_status":"open","n":"1","parents":[],"plain":"Nitrogenase must add four electrons and protons to its metal cluster before nitrogen gas can bind and be broken. Which metal atom grabs the nitrogen and what the cluster looks like at that moment are uncertain.","posed_since":"","precise":"For the E4 (Janus) state of FeMoco, determine the positions of the four added hydrogens (bridging hydrides, protonated sulfides), the site of N2 binding (Fe2/Fe6 face, Mo, or after sulfide displacement), and the energetics of reductive elimination of H2 coupled to N2 binding. An answer is a structure and mechanism consistent with ENDOR/EPR data and with converged electronic-structure energetics.","problem_ref":null,"references":"","settled_by":"Spectroscopic and freeze-quench structural data on trapped intermediates matched by converged calculations of the competing binding modes.","status_note":"","title":"Where and how nitrogen binds to the nitrogenase cofactor","topic_ref":"5b12fe0152e986aca51273bb7ce568020ca70d05bd813b448aa1fd3ffa1d742c"},"content_withheld":"false","files":[],"attested":{"attestation":{"batch":null,"client_id":null,"id_token_sha256":null,"kind":"platform"},"record_hash":"4673c437f55e43f7de741835c471e41986328655ac3b2a8abfe1db320de31a51","schema":"pubphys.attested/1"},"id_token":null,"id_token_withheld":"false","envelope":{"attested_hash":"4b4f5f4cc95b4f3e176eaac09b92c76c806f7a60e2b6d197deaaae7d80c77167","platform_signature":{"key_id":"c6afc19b31429869751f06879c75cd64ea92654423d15b44be775bf1310a60da","sig":"JUVytmypeNMDhk9vcxLp2H-5Yza-X6pMhbx5NAVikUWS1AkWnsHGUmOncbn28449v0ZcX7LRZ1f6CJHb_z33CA"},"schema":"pubphys.envelope/1"},"ots":{"attested":["AE9wZW5UaW1lc3RhbXBzAABQcm9vZgC_ieLohOiSlAEIS09fTMlbTz4XbqrAm5LHbIBvemDittGX3qqufYDHcWfxIEtMh8yQ-RDRmsN-jfg7u6N-yp8502F6ZAr1kkY5fmgCCPAgz7lhnp0jG95pFSvd2_6cGV8CtAr9lzRxAMbReQjKYe8I8CBbNruxpx2xAXBHvaKGioXQAG7BZbr2NyCtniXsIg7LIgjwIDjIxA36HBshHXchUifi0FBgSKzzBC-hqO12pqjEsGNQCPEgJnTaCWDUHc047Un9gxxz9vii53PpzzIvdFcEppqSbE0I8CDHtrxowIx6FimPIvh69X07RB6BXKqX3RN5DhqUvhKq9AjxIA8Mvjd87ZtpxfGaeH-X_AyCvFZCuMJEG65d8GBPbZfZCPEgsPKCRq0mf_8ht2OmXvMNFmmqll5AzUTeepEqQILv1TUI8CDcBxRb7fDApTWNNvjZ_r2lDpQT60DYte3pStKjciGgOAjwIK9SXvHBwgJE5CknXtp2lOqKcbHlJ-ncP9sNjnTHUJsmCPEgytIBB3x7TFDT-XqOV-9vpqw1NKoAu0dhtsCbAMql2L0I8CCtNZgmApuKULvPC9nsMCaM05arV-F3o5FTlnJCnRvdMgjwIBGYo5Vxy-d7U5zKv5vp3Ii7hJPH_BDLCzA13c47p9q7CP_wCHbghVgOFMuNCPAQPZK2lHbTMqcCYUotzOQcmQjwIJ7ruRIs6HnufKxCpmLUgeUl0aUu0ov4_3PqcppS-bddCPEgI530vHzdwz_3o9jYQ3WablSaV64Wfn7Pmby5WLyI-A0I8QRqwKw78AhITF7Tr3a1xP8Ag9_jDS75DI4sK2h0dHBzOi8vYm9iLmJ0Yy5jYWxlbmRhci5vcGVudGltZXN0YW1wcy5vcmcI8CBXWeNiWFWltfHE0nkDXsVKIykUUwuObhsnN-2fvBt98gjwIM5Vf7j68sOFgVysYMc5S09Hho-fqxYW3oQ4oKUX7JpPCPAgaKHHQB3sdd9w1hu4NMh7vmb-p0X2r4uyVKKCj1G7S28I8SAW2Mg6Bzi9ygjrrjIYB7w90fwMcxH24SsqLmzPyAMGfwjwIMQ863zlveYIpNKWVrZaDOa4vowwoOg2CcTW8gXtxK3dCPEgrZxGGAf-t8jhz_eLMUyBN1IhNrdYDRcNHWvQcwNG9woI8CCVOUHG1YXudbaFIgUYvrCIevRWvb2ubhQAo-z0duD0rQjxIDBeKerVGkO3J2eXDwdraSioXbIWsdIPEN1s8pIwJIS2CPAgV2kB3n3JZ5HwBQjpRL1CWKnqvdCjZk3RcALk6N2wHigI8CB-wbljNlfqElD25uZkNg6taS81YDRU2vfMqGVQ420O9AjxWQEAAAABJM43UU6MLMRrPlCUqossvu1q_knons5yylyWTgxZzWUAAAAAAP7___8CLgABAAAAAAAWABQrYt3E7OllKRCEmL3FL589l78_5wAAAAAAAAAAImog8ATiyw4ACAjxILmbcIO7ixv3pCtwUMZ1VQGKus54F9lZ8-w5ECD5ZuiICAjwIG6ynCyFTLPE7Rgk-0ZGCtBDyqNQx8YvaSZNHy8D8p8jCAjwIMX8zoD2Be1YWvcx_bexVPRmBX0hK-do8b8t9SarimQVCAjxIG6XtpqIEclLlhzcK1LVjqoPGrjjmLnyno8tcETMzECECAjwIF6mlfXOFY51JhrK4eze8zQlKV75OYZYqbtyi8BWxmAsCAjwIKKUcJmVoz-u-geiRV_-ZHdF0VEPXaEPFWf_JX_0e7G2CAjxIGMeD0Z5QWNKBcAEpHht007FFIILW6aSJ8f3CMkKNsneCAjwIBFr6Je8rkB7kwhPGbq0yRgFwZsvW9JhY5sXDRtrq0QwCAjwIAHAwXaus-yUgKICJB5mVvx8VZuJgjwnpb0qWPg1pi6DCAjxIAJhb8cefkVHTAMymdsxa-_i-tUua62LcpALQnPG7R9_CAjwIDvsFqBhPfAL6TCtXZd7gBWmMpx6iiZ-Ug0Jf_LlcstgCAjwIDJRBX6Q0Vge-ra-k1_g76yJWTEp6WWF13I3JpTnsZPJCAjwILykZCbkC9VQgl5gytPKKir6T9nTvKR6y05iWSHBTQgzCAgABYiWDXPXGQED45c78Ah-sGbmQAwfZwjwEAYqxtHnZiPZnJEGPx_JvKsI8SDXkqDg17YsZIrEz5acRx7mBXCKW5mKNgRYnw_-U2cpDgjxBGrArDrwCEfK6Mono428_wCD3-MNLvkMji4taHR0cHM6Ly9hbGljZS5idGMuY2FsZW5kYXIub3BlbnRpbWVzdGFtcHMub3JnCPAg10nyJ6Jn4kshQQQCKE0Lvj3hBC6nLRBVIzaPV4eh8VwI8CCQaAqWlxEbac_XOq1MOlf9iOKHEsxXspzbjK0vLpy8BwjwII3iRbRXUcDxrhAdkZmFlI15nySCSEZ7g922tF1Xln5DCPEgYYvZ1L4v4rPu_UqLfcHPtwSb2OH2GhPgVcVhhH6DpkQI8CDZCIoR20sC-uchP40oAmcLm9EJFV7xzwplj54AeVadXQjxILvLBjPFpCoz29m9Yf2qRnV7PI9GqxE8ozVQfk9QwCqrCPEgZXcMudvEoB_MJ8tqOW2PLTKAIeQBnE-1bfzptRc2gR8I8VkBAAAAAfXq-bmxWqOz7B5wKkwjzHGv2R4s7Y0Z76LqXWYNhmz0AAAAAAD-____Av0cAAAAAAAAFgAUl-skmdZDtn7Hnub6mF4HP2uVn7oAAAAAAAAAACJqIPAE4MsOAAgI8CBDmQsbEXc46Hu8DivhCBM1LGExa4TTM-wo7HpoCVjBTQgI8CCty5vkQtPHzzs8xqArOYhVSjgaDoG1ou3cojv5_P7wQQgI8SDQWB0kdXCTwbk-TqTt0QfRMyzDNLOtU1XXFgvGSRlW8wgI8CC2PKa_QCIBZtrXodq1GdEzppISMsMRDOnzr2gFjLKOAAgI8CCwQ_QF67bcFk_uJ2s0VCZmf5PGIjTmTCpt_qnVY5p_8AgI8SAbzyx7z-oxPMju6PvtQhzrJIQyH2ZAc9oyLuYemzbmZQgI8CC5bh7J74uUk91r8LwybHQFCNBC3PWeuwtMCCNFKWj19AgI8SAf4R2TUNPjaX_PWDUImpoITK0JoW89zaMKnD1wlEEi8AgI8SC2vNmdm7eyP5Ytl1yTzT0njliMdALl1ViKG-s3bG2zswgI8CCpbIVNz1GFVXdJk2zd82hBUoNFFemUPBt7IJdWEOciPwgI8CBtYdRQcrtX1FtCm1SxF7sx-5cO04U8KzbJh8fqBkyReAgI8CBHs2LGmX6gI-lV7yp1RrV7_tGfdiBAwnEFF6B6yXHzAQgI8CATotVmbXBMAixhZ-QKy5Q4lR0FusdA_wcIC1TbB7GmxAgIAAWIlg1z1xkBA-KXOw"],"envelope":["AE9wZW5UaW1lc3RhbXBzAABQcm9vZgC_ieLohOiSlAEIDIGxGAmj9VpYvXLQij2o6NiDI4fAGSvMIltpF9vvMZvwIAyG7M6PYsyzI1O9HCsLkL9qIzh1iJOfJm8TSMQSk_9lCPEgnrimjamfGngyZKLZkZU7MGXTFQht1iGuBCpZSx8HRKwI8CAFm9AKBVI3GbE9tVp2ARtbPGPMwT9KOxaDgsJSQX2uIwjxIII8CG1OD0iLOJA9IIuyHxaOmLNKr3Pd-orWulwTsKSmCPEgNEUc_F6ktAagdk6E0YYnsUqC9pt3K2VrR-uJIc2_i_AI8CD20EgCzlmnqHeccy6BW9Ifsp_dRylp022jVxy290j6EgjxIBButcXheltB9r5q_SiVaYiPmqtX1_20ZNerwgnGiSE_CPEgoIuFmZyvZNn9xG6qppiK_mJT40FHqHfjKvxdbYaYwQAI8CDTURm5psgkpndkZlRIia_PxrzHEbIi4fWR0w8YEe9YLAjwIMIc4xq7iJ2YP27EtWYUvEm4-TQD7cayDq2XvZh74n3BCPAg3v2QXfNkDO6Xzu9061umH0rhjRfHBKza3uG2nPQbpd8I8CCtNZgmApuKULvPC9nsMCaM05arV-F3o5FTlnJCnRvdMgjwIBGYo5Vxy-d7U5zKv5vp3Ii7hJPH_BDLCzA13c47p9q7CP_wCHbghVgOFMuNCPAQPZK2lHbTMqcCYUotzOQcmQjwIJ7ruRIs6HnufKxCpmLUgeUl0aUu0ov4_3PqcppS-bddCPEgI530vHzdwz_3o9jYQ3WablSaV64Wfn7Pmby5WLyI-A0I8QRqwKw78AhITF7Tr3a1xP8Ag9_jDS75DI4sK2h0dHBzOi8vYm9iLmJ0Yy5jYWxlbmRhci5vcGVudGltZXN0YW1wcy5vcmcI8CBXWeNiWFWltfHE0nkDXsVKIykUUwuObhsnN-2fvBt98gjwIM5Vf7j68sOFgVysYMc5S09Hho-fqxYW3oQ4oKUX7JpPCPAgaKHHQB3sdd9w1hu4NMh7vmb-p0X2r4uyVKKCj1G7S28I8SAW2Mg6Bzi9ygjrrjIYB7w90fwMcxH24SsqLmzPyAMGfwjwIMQ863zlveYIpNKWVrZaDOa4vowwoOg2CcTW8gXtxK3dCPEgrZxGGAf-t8jhz_eLMUyBN1IhNrdYDRcNHWvQcwNG9woI8CCVOUHG1YXudbaFIgUYvrCIevRWvb2ubhQAo-z0duD0rQjxIDBeKerVGkO3J2eXDwdraSioXbIWsdIPEN1s8pIwJIS2CPAgV2kB3n3JZ5HwBQjpRL1CWKnqvdCjZk3RcALk6N2wHigI8CB-wbljNlfqElD25uZkNg6taS81YDRU2vfMqGVQ420O9AjxWQEAAAABJM43UU6MLMRrPlCUqossvu1q_knons5yylyWTgxZzWUAAAAAAP7___8CLgABAAAAAAAWABQrYt3E7OllKRCEmL3FL589l78_5wAAAAAAAAAAImog8ATiyw4ACAjxILmbcIO7ixv3pCtwUMZ1VQGKus54F9lZ8-w5ECD5ZuiICAjwIG6ynCyFTLPE7Rgk-0ZGCtBDyqNQx8YvaSZNHy8D8p8jCAjwIMX8zoD2Be1YWvcx_bexVPRmBX0hK-do8b8t9SarimQVCAjxIG6XtpqIEclLlhzcK1LVjqoPGrjjmLnyno8tcETMzECECAjwIF6mlfXOFY51JhrK4eze8zQlKV75OYZYqbtyi8BWxmAsCAjwIKKUcJmVoz-u-geiRV_-ZHdF0VEPXaEPFWf_JX_0e7G2CAjxIGMeD0Z5QWNKBcAEpHht007FFIILW6aSJ8f3CMkKNsneCAjwIBFr6Je8rkB7kwhPGbq0yRgFwZsvW9JhY5sXDRtrq0QwCAjwIAHAwXaus-yUgKICJB5mVvx8VZuJgjwnpb0qWPg1pi6DCAjxIAJhb8cefkVHTAMymdsxa-_i-tUua62LcpALQnPG7R9_CAjwIDvsFqBhPfAL6TCtXZd7gBWmMpx6iiZ-Ug0Jf_LlcstgCAjwIDJRBX6Q0Vge-ra-k1_g76yJWTEp6WWF13I3JpTnsZPJCAjwILykZCbkC9VQgl5gytPKKir6T9nTvKR6y05iWSHBTQgzCAgABYiWDXPXGQED45c78Ah-sGbmQAwfZwjwEAYqxtHnZiPZnJEGPx_JvKsI8SDXkqDg17YsZIrEz5acRx7mBXCKW5mKNgRYnw_-U2cpDgjxBGrArDrwCEfK6Mono428_wCD3-MNLvkMji4taHR0cHM6Ly9hbGljZS5idGMuY2FsZW5kYXIub3BlbnRpbWVzdGFtcHMub3JnCPAg10nyJ6Jn4kshQQQCKE0Lvj3hBC6nLRBVIzaPV4eh8VwI8CCQaAqWlxEbac_XOq1MOlf9iOKHEsxXspzbjK0vLpy8BwjwII3iRbRXUcDxrhAdkZmFlI15nySCSEZ7g922tF1Xln5DCPEgYYvZ1L4v4rPu_UqLfcHPtwSb2OH2GhPgVcVhhH6DpkQI8CDZCIoR20sC-uchP40oAmcLm9EJFV7xzwplj54AeVadXQjxILvLBjPFpCoz29m9Yf2qRnV7PI9GqxE8ozVQfk9QwCqrCPEgZXcMudvEoB_MJ8tqOW2PLTKAIeQBnE-1bfzptRc2gR8I8VkBAAAAAfXq-bmxWqOz7B5wKkwjzHGv2R4s7Y0Z76LqXWYNhmz0AAAAAAD-____Av0cAAAAAAAAFgAUl-skmdZDtn7Hnub6mF4HP2uVn7oAAAAAAAAAACJqIPAE4MsOAAgI8CBDmQsbEXc46Hu8DivhCBM1LGExa4TTM-wo7HpoCVjBTQgI8CCty5vkQtPHzzs8xqArOYhVSjgaDoG1ou3cojv5_P7wQQgI8SDQWB0kdXCTwbk-TqTt0QfRMyzDNLOtU1XXFgvGSRlW8wgI8CC2PKa_QCIBZtrXodq1GdEzppISMsMRDOnzr2gFjLKOAAgI8CCwQ_QF67bcFk_uJ2s0VCZmf5PGIjTmTCpt_qnVY5p_8AgI8SAbzyx7z-oxPMju6PvtQhzrJIQyH2ZAc9oyLuYemzbmZQgI8CC5bh7J74uUk91r8LwybHQFCNBC3PWeuwtMCCNFKWj19AgI8SAf4R2TUNPjaX_PWDUImpoITK0JoW89zaMKnD1wlEEi8AgI8SC2vNmdm7eyP5Ytl1yTzT0njliMdALl1ViKG-s3bG2zswgI8CCpbIVNz1GFVXdJk2zd82hBUoNFFemUPBt7IJdWEOciPwgI8CBtYdRQcrtX1FtCm1SxF7sx-5cO04U8KzbJh8fqBkyReAgI8CBHs2LGmX6gI-lV7yp1RrV7_tGfdiBAwnEFF6B6yXHzAQgI8CATotVmbXBMAixhZ-QKy5Q4lR0FusdA_wcIC1TbB7GmxAgIAAWIlg1z1xkBA-KXOw"]},"log":{"leaf_index":"849","tree_size":"2123","proof":["51e0b5fbeb94ee0abd826c7bf5f74cf5458246a549f93cff2c03a1365deee748","0decdac43ce3b056b1be271ff84a1aa4559f7214492ee226d651edc7f2b06070","765f27f529d72ccb7aa2fe7e9d1db75850d70765155eb08d3b8ec194b403dd61","2162cfd9bbe9b663391b18e1bbeb877173d12c5baa444c30e5c3b70249ffc26d","f3ae5df6f08fdfe9b8aca908569be167cf35aad37cf05a45e08c6677b6a7862e","d7ed69775f9f907773b782007bc69ba80341e9a021c8bd9af082969a3b170987","5ecc83ce2a0a6a0b32bd81f4758ee72170756fb346ac2b8fc45c2c1e3c306352","3da8ba69a14fe8a80e419cbc158ef45e4ebf9476aa4342fe43b8a2fe83bd6988","cba222c71b49d79b667b9eb145f4a2927b31dafcf0f9407638f19a25d0726210","14108339881e6713df1e6d34582069c609e80460dc60638e661361aa4403ff51","ec3fa24572dee1df200ec665f3e3244d925b199f0f84e225b820c3b66bd7b094","addcf2e0ff7974e4aa99af5e30d409f90d9f4311a4d85f5baf3e125eeb45b4c1"],"checkpoint":"pubphys.com/log/v1\n2123\npXVcluz7BF4vgChQs6lShuU0386gCH0RxrC1sxyE3rI=\n\n— pubphys.com/log/v1 JFPwIOLzsUD22XoTVyTfk/FMVj/u3f3DxZk4V/Ws10KVSS+8WQSwhunQ//EAhyS9Td5CyM0E++85GMezmKd8240j9Q8=\n","promise":null},"orcid_key_evidence":[],"attesting":null}