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How the frequency of such contacts translates into gene activity is unknown.","posed_since":"","precise":"For an enhancer-promoter pair at genomic separation $s$, relate contact probability $p_c(s)$ (or contact rate) to transcriptional burst frequency, and identify the mechanism behind the reported nonlinear dependence of expression on $p_c$ (threshold, multi-step activation, or action at a distance through a condensate). Answer: a quantitative model predicting expression across inserted enhancer positions.","problem_ref":null,"references":"","settled_by":"Systematic enhancer-repositioning experiments with simultaneous live measurement of contacts and bursting that match one model.","status_note":"Enhancer-repositioning experiments (2022) reported a nonlinear dependence of expression on contact probability; its mechanism is unresolved.","title":"How enhancer-promoter contact frequency sets 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