Revision 1

Accepted · first revision · record

Title: Predict condensate phase diagrams from protein sequence
Plain: A disordered protein's amino-acid sequence decides at what concentration it forms droplets. A method that predicts this concentration from sequence, across salt and temperature, would connect sequence to physics directly.
Precise: For intrinsically disordered regions of 50 to 500 residues, predict the saturation concentration $c_{\mathrm{sat}}(T,\mathrm{salt})$ and the dense-phase concentration from sequence alone, within a factor of 2 for $c_{\mathrm{sat}}$, across sequence variants with altered aromatic and charge patterning (stickers-and-spacers framework). Answer: a model validated on blind variants and on two-component mixtures.
Settled by: Blind predictions of $c_{\mathrm{sat}}$ for newly designed sequence variants compared with measured phase diagrams.
Kind: well-posed
Answer type: value
References: (empty)
Literature status: partially-resolved
Status note: Residue-level coarse-grained models reproduce $c_{\mathrm{sat}}$ trends within families of disordered regions; accuracy for arbitrary sequences and mixtures is not established as of 2026, to this survey's knowledge.
Posed since: (empty)